GlycoBioinformatics

• Kiyoko F. Aoki-Kinoshita,
• Frédérique Lisacek,
• Niclas Karlsson,
• Daniel Kolarich and
• Nicolle H. Packer

Beilstein J. Org. Chem. 2021, 17, 2726–2728, doi:10.3762/bjoc.17.184

• knowledge. Most structural data at this stage is generated by analytical approaches, such as mass spectrometry (MS), high-pressure liquid chromatography (HPLC), and capillary electrophoresis (CE). The articles by Phung et al. [9] and by Lippold et al. [10] suggest ways of combining and customising available

The fluorescence of a mercury probe based on osthol

• Guangyan Luo,
• Zhishu Zeng,
• Lin Zhang,
• Zhu Tao and
• Qianjun Zhang

Beilstein J. Org. Chem. 2021, 17, 22–27, doi:10.3762/bjoc.17.3

• irreversible nerve damage. Therefore, it is very important to develop a highly sensitive and selective method for mercury detection. At present, the detection of mercury mainly includes atomic absorption and atomic emission spectrometry [4], inductively coupled plasma mass spectrometry [5][6], and capillary

• electrophoresis [7]. However, the application of these analytical methods in mercury detection is limited due to the complex sample pretreatment procedures, expensive instruments, and other factors [8][9]. Fluorescent-probe instruments have been widely used due to the advantages of the simple operation, low cost

Leveraging glycomics data in glycoprotein 3D structure validation with Privateer

• Haroldas Bagdonas,
• Daniel Ungar and
• Jon Agirre

Beilstein J. Org. Chem. 2020, 16, 2523–2533, doi:10.3762/bjoc.16.204

• and the chain length. However, the in-depth analysis of a glycan typically requires the integration of complementary analytic techniques, such as nuclear magnetic resonance (NMR) and capillary electrophoresis (CE). Nevertheless, depending on the sample, advanced mass spectrometry techniques can be

Clustering and curation of electropherograms: an efficient method for analyzing large cohorts of capillary electrophoresis glycomic profiles for bioprocessing operations

• Ian Walsh,
• Matthew S. F. Choo,
• Sim Lyn Chiin,
• Amelia Mak,
• Shi Jie Tay,
• Pauline M. Rudd,
• Yang Yuansheng,
• Andre Choo,
• Ho Ying Swan and
• Terry Nguyen-Khuong

Beilstein J. Org. Chem. 2020, 16, 2087–2099, doi:10.3762/bjoc.16.176

• critical process parameters that control the glycosylation critical quality attributes. The advances made in protocols for capillary electrophoresis-laser-induced fluorescence (CE-LIF) measurements of antibody N-glycans have increased the potential for generating large datasets of N-glycosylation values

• optimization study. The key advantage of this computational approach is that all runs can be analyzed simultaneously with high accuracy in glycan identification and quantitation and there is no theoretical limit to the scale of this method. Keywords: capillary electrophoresis; clustering; data analysis

• description of the glycans, their relative abundance, and most importantly be high-throughput in terms of quantity, comprehensiveness, and speed of data generation. Capillary electrophoresis-laser-induced fluorescence (CE-LIF) is a glycomic analytical technology that has been adapted for automated and high

Graphitic carbon nitride prepared from urea as a photocatalyst for visible-light carbon dioxide reduction with the aid of a mononuclear ruthenium(II) complex

• Kazuhiko Maeda,
• Daehyeon An,
• Ryo Kuriki,
• Daling Lu and
• Osamu Ishitani

Beilstein J. Org. Chem. 2018, 14, 1806–1812, doi:10.3762/bjoc.14.153

• formate generated in the liquid phase was analyzed via a capillary electrophoresis system (Otsuka Electronics Co., Model CAPI-3300). XRD patterns of g-C3N4 synthesized at different temperatures. A broad peak at around 22 degree, indicated by #, in XRD patterns originated from a glass folder for the

Molecular recognition of N-acetyltryptophan enantiomers by β-cyclodextrin

• Spyros D. Chatziefthimiou,
• Mario Inclán,
• Petros Giastas,
• Athanasios Papakyriakou,
• Konstantina Yannakopoulou and
• Irene M. Mavridis

Beilstein J. Org. Chem. 2017, 13, 1572–1582, doi:10.3762/bjoc.13.157

• chromatography [24], or in capillary electrophoresis [25]. Results and Discussion NMR studies In deuterium oxide (D2O), each of the NAcTrp enantiomers induced significant chemical shift displacements (shielding) in the 1H NMR signals of the β-CD cavity protons, namely H3 (near the wider, secondary side) and H5

NMR reaction monitoring in flow synthesis

• M. Victoria Gomez and
• Antonio de la Hoz

Beilstein J. Org. Chem. 2017, 13, 285–300, doi:10.3762/bjoc.13.31

• ), capillary electrophoresis (CE), capillary LC–NMR (CapLC–NMR), capillary electrochromatography-NMR (CEC–NMR), capillary isotachophoresis (cITP) and size-exclusion chromatography-NMR (SEC–NMR) [28]. Hyphenation is another important field in which separation and analytical techniques are combined. Hyphenation

Phosphated cyclodextrins as water-soluble chiral NMR solvating agents for cationic compounds

• Cira Mollings Puentes and
• Thomas J. Wenzel

Beilstein J. Org. Chem. 2017, 13, 43–53, doi:10.3762/bjoc.13.6

• . Unfortunately, CM-CDs with high DS are not commercially available and investigators wishing to use these compounds in chiral NMR applications would have to synthesize and purify them. Phosphated CD derivatives (P-CDs, Figure 1) have been utilized as effective enantioselectors in capillary electrophoresis [45

New synthetic strategies for xanthene-dye-appended cyclodextrins

• Milo Malanga,
• Andras Darcsi,
• Mihaly Balint,
• Gabor Benkovics,
• Tamas Sohajda and
• Szabolcs Beni

Beilstein J. Org. Chem. 2016, 12, 537–548, doi:10.3762/bjoc.12.53

• inexpensive dyes, we prepared rhodamine- and fluorescein-appended cyclodextrins. The compounds were characterized by NMR and IR spectroscopy and MS spectrometry, their UV–vis spectra were recorded at various pH, and their purity was determined by capillary electrophoresis. Two potential models for the

Supramolecular structures based on regioisomers of cinnamyl-α-cyclodextrins – new media for capillary separation techniques

• Gabor Benkovics,
• Ondrej Hodek,
• Martina Havlikova,
• Zuzana Bosakova,
• Pavel Coufal,
• Milo Malanga,
• Eva Fenyvesi,
• Andras Darcsi,
• Szabolcs Beni and
• Jindrich Jindrich

Beilstein J. Org. Chem. 2016, 12, 97–109, doi:10.3762/bjoc.12.11

• that the position of the cinnamyl group plays an important role in the intermolecular complex formation. Keywords: capillary electrophoresis; cyclodextrin derivatives; mono-cinnamyl; regioisomers; supramolecular structures; Introduction Supramolecular polymers (SP) are aggregates of monomer units

• prepare the two regioisomers of Cin-α-CD in gram-scale which was required for the detailed characterization of their aggregation ability and for their application in capillary electrophoresis (CE). The monosubstituted products were characterized by ESI mass spectrometry, 1H NMR and 13C NMR spectroscopy

• capillary electrophoresis. The influence of the addition of 2-O-Cin-α-CD and 3-O-Cin-α-CD to the background electrolyte (BGE) and its impact on the effective mobilities of eighteen selected analytes were tested. Nine analytes were in the form of cations (aniline, antipyrine, L-histidine, DL-tyrosine, DL

Synthetic strategies for the fluorescent labeling of epichlorohydrin-branched cyclodextrin polymers

• Milo Malanga,
• Mihály Bálint,
• István Puskás,
• Kata Tuza,
• Tamás Sohajda,
• László Jicsinszky,
• Lajos Szente and
• Éva Fenyvesi

Beilstein J. Org. Chem. 2014, 10, 3007–3018, doi:10.3762/bjoc.10.319

• techniques and capillary electrophoresis. The applied synthetic routes were evaluated based on the molecular weight, cyclodextrin content of the products and the efficiency of labeling. Keywords: coumarin; fluorescein; functionalized monomers and polymers; nitrobenzofurazan; rhodamine; Introduction

• around 0.01% w/w by capillary electrophoresis (CE), the amount of dye covalently attached to the polymer is between 0.45–0.5%. As previously mentioned, the strategy based on the post-branching modification (Scheme 1, upper part) can include a supplementary step for the modulation of the properties of the

Synthesis of modified cyclic and acyclic dextrins and comparison of their complexation ability

• Kata Tuza,
• László Jicsinszky,
• Tamás Sohajda,
• István Puskás and
• Éva Fenyvesi

Beilstein J. Org. Chem. 2014, 10, 2836–2843, doi:10.3762/bjoc.10.301

• capillary electrophoresis (CE) using model guest compounds. In some cases cyclodextrins and their open-ring analogs (acyclodextrins) show similar complexation abilities, while with other guests considerably different behavior was observed depending on the molecular dimensions and chemical characteristics of

• or even better enantioresolution compared to their cyclic counterparts commonly utilized in chiral separations [8]. One of the most effective techniques to explore if there are interactions between guests and hosts – particularly in the case of ionizable guest molecules – is capillary electrophoresis

Integrating reaction and analysis: investigation of higher-order reactions by cryogenic trapping

• Skrollan Stockinger and
• Oliver Trapp

Beilstein J. Org. Chem. 2013, 9, 1837–1842, doi:10.3762/bjoc.9.214

• ][17]. Here, the catalyst is immobilized or dissolved in an inert stationary phase and coated on the inside of fused-silica capillaries, which are then installed in a gas chromatograph (Figure 1a), capillary electrophoresis (Figure 1b) or liquid separation instrument (Figure 1c). In addition, the

• an immobilized or in an inert stationary phase dissolved catalyst. The use of reactive capillaries a) in on-column reaction GC, b) in on-column reaction capillary electrophoresis, c) as a capillary reactor to perform flow-through experiments, and d) to obtain catalytically active glassware. The

Molecular recognition of organic ammonium ions in solution using synthetic receptors

• Andreas Späth and
• Burkhard König

Beilstein J. Org. Chem. 2010, 6, No. 32, doi:10.3762/bjoc.6.32